Answer
$3\sqrt {9u^{2}+1}+\sqrt {u^{2}+1}$
Work Step by Step
$G(x)$ = $\int_{-u}^{3u}\sqrt {x^{2}+1}dx$ = $\int_{0}^{3u}\sqrt {x^{2}+1}dx$ - $\int_{0}^{-u}\sqrt {x^{2}+1}dx$
apply chain rule with FTC then get
$G'(x)$ = $3\sqrt {9u^{2}+1}+\sqrt {u^{2}+1}$