Answer
$16x^4-8x^2+1$
Work Step by Step
Using $(a\pm b)^2=a^2\pm2ab+b^2$ or the square of a binomial, the square of the given expression, $(
4x^2-1
)^2,$ is
\begin{array}{l}\require{cancel}
(4x^2)^2-2(4x^2)(1)+(1)^2
\\\\=
16x^4-8x^2+1
.\end{array}