God
God is the Christian God, who in this play (and in the original biblical story) commands Abraham to kill his son, Isaac. God communicates with Abraham first through one of his angels, and later directly, when he blesses Abraham for expressing his willingness to sacrifice his son and Isaac for his willingness to be sacrificed. God's blessing includes a promise to "multiply [Abraham and Isaac's] seed / As thick as stars be in the sky," so that both have a long and fruitful legacy.
Abraham
Abraham is the "founding father" of the three Abrahamic religions--Judaism, Christianity, and Islam. In the Hebrew Bible, he formed the Covenant with God--a special relationship and promise between God and the Jewish people. In Christianity, Abraham is looked to as a model of pure faith. God chooses Abraham and sends an angel to command him to sacrifice his son, Isaac. Although Abraham is conflicted, he feels that he has no choice, since his love and loyalty to his God come before his love for his son. He goes to sacrifice Isaac, but is stopped by the angel and told to sacrifice a ram instead. God, seeing Abraham's willingness to sacrificed his beloved son and Isaac's understanding, blesses Abraham and Isaac with many offspring and a long legacy.
Isaac
Isaac is Abraham's son and the only son born to Abraham by his wife, Sarah. Upon hearing that his father plans to sacrifice him, Isaac begs for his life; however, when he finds out that it is God's will, he accepts his fate.
Angel
The angel is a messenger of God who communicates God's will to Abraham. When Abraham goes to sacrifice Isaac, the angel stops him by snatching his sword away and tells him to sacrifice a ram instead.
Doctor
The Doctor is a stock figure in medieval drama who, in this play, appears in the epilogue to summarize the story and point out the moral: that people should obey God's commandments and not complain about the life God wills for you, whether it is good or bad.