Chapter 14 - From DNA to Protein: Gene Expression - 14.4 - Eukaryotic Pre-mRNA Transcripts Are Processed prior to Translation - 14.4 Recap - Learning Outcomes - Page 301: 1

In nucleic acid hybridization, a sample of DNA containing the target gene is denatured to separate the two strands. Immediately after, the single-stranded mRNA incubated alongside the denatured DNA. In the case that the mRNA has a base sequence that is complementary to the target DNA, a double helix forms between the mRNA and the target DNA by hydrogen bonding between the bases. Since the two strands have different origins, the resulting double-stranded region is referred to as a hybrid. This allows biologists to identify and pinpoint the relevant introns within eukaryotic genes.

In nucleic acid hybridization,a sample of DNA containing the target gene is denatured to separate the two strands. Immediately after, the single-stranded mRNA incubated alongside the denatured DNA. In the case that the mRNA has a base sequence that is complementary to the target DNA, a double helix forms between the mRNA and the target DNA by hydrogen bonding between the bases. Since the two strands have different origins, the resulting double-stranded region is referred to as a hybrid.