Answer
GFP (Green fluorescent protein) is a bioluminescent protein found in the jellyfish (Aequorea victoria), commonly called the crystal jelly. Normally, this jellyfish is transparent. However, when disturbed, special cells in the jellyfish release a fluorescent protein called aequorin. Aequorin fluoresces with a green color. This protein can be isolated from jellyfish and can be used as a molecular tag. These tags can be generated for almost any protein within the cell, revealing not only its cellular location but also how its distribution within the cell may change as a result of a response to its environment. GFP labeled antibody can be used to identify the cellular location of the actin proteins in a human cell,such as cancer cell.
Work Step by Step
GFP (Green fluorescent protein) is a bioluminescent protein found in the jellyfish (Aequorea victoria), commonly called the crystal jelly. Normally, this jellyfish is transparent. However, when disturbed, special cells in the jellyfish release a fluorescent protein called aequorin. Aequorin fluoresces with a green color. This protein can be isolated from jellyfish and can be used as a molecular tag. These tags can be generated for almost any protein within the cell, revealing not only its cellular location but also how its distribution within the cell may change as a result of a response to its environment. GFP labeled antibody can be used to identify the cellular location of the actin proteins in a human cell,such as cancer cell.
