Answer
If a polar amino acid in this catalytic center is replaced with non-polar amino acid, then generation of nucleophile or an electrophile (charged species) would not be takes place.
Work Step by Step
An abrupt change in the sequence of DNA (deoxyribonucleic acid) due to physical or chemical factors is called a mutation. There are several types mutations can alter the DNA sequence, namely deletions, insertion and point mutations, etc. These mutations change the sequence of proteins (one amino acid in a protein is replaced by another amino acid) and this results change in properties and functions of proteins too.
Enzymes are nothing but proteins, but all proteins are not enzymes. So, the properties of enzyme can be changed by the replacement of one amino acid with another particular amino acid. During this process the activity of the enzyme will be enhanced or it will be lost. The active site of enzyme has a combination of amino acids, which together act as a catalytic center.
If a polar amino acid in this catalytic center is replaced with non-polar amino acid, then generation of nucleophile or an electrophile (charged species) would not be takes place. This results the inhibition of the activity of the enzyme, because enzyme could not to the substrate.
