Answer
No, though the convention is write the sequence starting with the 5' end. You need more information about the chemical structure/which end has a phosphate sticking out.
Work Step by Step
Review the information in Concept 16.1 for diagrams that will show how 3' and 5' are defined. A phosphate group on the 5' carbon would indicate the 5' end and an -OH group on the 3' carbon would indicate the 3' end.
